D Amino Acid Oxidase (DAO)
(D-Amino-Acid Oxidase (DAO))
Reactivity
Pig
Host
Sheep
Clonality
Polyclonal
Conjugate
This D Amino Acid Oxidase antibody is un-conjugated
Application
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
This antibody detects porcine D-amino-acid oxidase. Immunoelectrophoresis give a single precipitin arc against purified and partially purified D-amino-acid oxidase [pig kidney].
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
Verified
DAO
Reactivity: Human
ELISA, IHC
Host: Goat
Polyclonal
unconjugated
Application Notes
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of D-Amino AcidOxidase [Pig Kidney] in a standard sandwich ELISA using Peroxidase conjugated affinitypurified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 20,000 to 1: 100,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, with 0.01 % Sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
Target
D Amino Acid Oxidase (DAO)
(D-Amino-Acid Oxidase (DAO))
This gene encodes the peroxisomal enzyme D-amino-acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino-acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known, it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino-acids accumulated during aging, or it may be a fossil enzyme without a current function.Synonyms: DAAO, DAMOX, DAO, DAO1, OXDA