Luciferase antibody

Catalog No. ABIN116768

The Rabbit Polyclonal anti-Luciferase antibody is suitable to detect Luciferase in samples from Vibrio fischeri. It has been validated for WB and EIA.

Quick Overview for Luciferase antibody (ABIN116768)

Target: Luciferase

Reactivity: Vibrio fischeri

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Luciferase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Luciferase [Photobacterium fischerii]

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/1,000-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 μg of Luciferase [Photobacterium fischerii]in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 16,000 of thereconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Luciferase

Target: Luciferase

Background: Synonyms: Alkanal monooxygenase alpha chain, LUXA

Gene ID: 553611

UniProt: P09140