Luciferase antibody

Catalog No. ABIN7212242

The Mouse Monoclonal anti-Luciferase antibody (Clone 6B8) (ABIN7212242) specifically detects Luciferase in WB and IF. The antibody is reactive with Firefly samples.

Quick Overview for Luciferase antibody (ABIN7212242)

Target: Luciferase

Reactivity: Firefly

Host: Mouse

Clonality: Monoclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 6B8

Product Details anti-Luciferase Antibody

Purpose: Mouse Anti-Luciferase Mouse Monoclonal Antibody (6B8)

Specificity: Luciferase protein detects endogenous levels of Luciferase.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Recombinant Protein of Luciferase of Luciferase

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:1000-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Luciferase

Target: Luciferase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Molecular Weight: 60kD

UniProt: P08659