Luciferase antibody (C-Term)

Catalog No. ABIN6254229

The Goat Polyclonal anti-Luciferase antibody (ABIN6254229) specifically detects Luciferase in WB. The antibody is reactive with Photinus pyralis samples.

Quick Overview for Luciferase antibody (C-Term) (ABIN6254229)

Target: Luciferase

Reactivity: Photinus pyralis

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Luciferase Antibody

Binding Specificity: C-Term

Purpose: Anti-Luciferase

Sequence: LTETTSAILI TPEGDDKPGA VGKVVPFFEA KVVDLDTGKT LGVNQRGELC VRGPMIMSGY VNNPEATNAL IDKDGWLHSG DIAYWDEDEH FFIVDRLKSL IKYKGYQVAP AELESILLQH PNIFDAGVAG LPDDDAGELP AAVVVLEHGK TMTEKEIVDY VASQVTTAKK LRGGVVFVDE VPKGLTGKLD ARKIREILIK AKKGGKSKL

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.

Characteristics: Firefly Luciferase

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for Luciferase

Target: Luciferase

Background:

Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Luc and Luciferin 4-monooxygenase antibody.