XDH antibody

Catalog No. ABIN116853

Cited in 1 publication. The Rabbit Polyclonal anti-XDH antibody (ABIN116853) specifically detects XDH in WB, IP and EIA. The antibody is reactive with Cow samples.

Quick Overview for XDH antibody (ABIN116853)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-XDH Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Xanthine Oxidase [Bovine Buttermilk].

Application Details

Application Notes: Suitable for immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 μg of XanthineOxidase [Bovine Buttermilk] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 100,000 to 1: 500,000 of the reconstitutionconcentration is suggested for this product. This product has been assayed by Immunhistochemical staining and was found to bereactive against bovine, human and mouse tissue at a dilution of 1: 5,000 to 1: 25,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.

References for anti-XDH antibody (ABIN116853)

Robenek, Hofnagel, Buers, Lorkowski, Schnoor, Robenek, Heid, Troyer, Severs: "Butyrophilin controls milk fat globule secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10385-10390, (2006) (PubMed).

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH

Background: Synonyms: Xanthine dehydrogenase/oxidase

Gene ID: 280960

NCBI Accession: NP_776397

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity