IL1RN antibody

Catalog No. ABIN116864

The Rabbit Polyclonal anti-IL1RN antibody is suitable to detect IL1RN in samples from Human and Monkey. It has been validated for WB, IHC (p), EIA, IHC (fro) and Func.

Quick Overview for IL1RN antibody (ABIN116864)

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-IL1RN Antibody

Purification: DEAE Chromatography.

Immunogen: Prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.

Application Details

Application Notes: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for usein Neutralizations, ELISA, Radioimmunoassays, Western blotting, Immunohistochemistryand Flow cytometry. Reactivity in other immunoassays is unknown. Note: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L]and TMB as a substrate. A working dilution range of 1: 400 to 1: 800 is suggested for thisapplication. This product has been assayed by ELISA against IL-1Receptor Antagonist usingHRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes atroom temperature. A working dilution range of 1: 500 to 1: 1,000 is suggested for thisproduct. For use in ELISA formats, this antibody is best used as the second antibody incombination with a monoclonal antibody as a capture antibody. This product has beenassayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry. This product has been assayed by radioimmunoassay againstantigen. A dilution of 1: 8,000 is suggested for this immunoassay. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL1RN / IL1RA

Background: IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein

Gene ID: 9606

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints