CAMP Responsive Element Binding Protein 1 (CREB1) (N-Term) antibody

Details for Product No. ABIN116948
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Synonyms creb, CREB1, Creb1, CREB, 2310001E10Rik, 3526402H21Rik, AV083133, Creb, Creb-1, creb1, zgc:55598
(76), (35), (27), (20), (19), (15), (14), (8), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(362), (241), (228), (75), (69), (68), (45), (9), (6), (6), (5), (1), (1), (1), (1)
(322), (54), (2), (1)
(11), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2)
Electrophoretic Mobility-Shift Assay (EMSA), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(283), (149), (146), (69), (60), (44), (29), (28), (13), (4), (2), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN116948
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Immunogen CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads. This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Alternative Name CREB1
Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.
Alternate names: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element- binding protein 1
Gene ID 1385
NCBI Accession NP_004370
UniProt P16220
Application Notes Western Blot: This antibody was assayed against a lysate of 1 x 10e6 Raji B cells byImmunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at adilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG(H&L) [Goat] (Cat#R1364HRP)ELISA: 1/5,000-1/25,000. Immunoprecipitation. Gelshift: This antibody was tested in Gel Supershift Assay and found to be reactive againstCREB-1 (p43) using 0.5-1.0 µl per assay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 2.56 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized (preferably in a desiccator) at 2-8°C and reconstituted (in aliquots) at -20°C.
Expiry Date 12 months
Supplier Images
anti-CAMP Responsive Element Binding Protein 1 (CREB1) (N-Term) antibody Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. Detection occurs using a 1:1,000 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Background publications Moreno, Beresford, Louis-Plence et al.: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999 (PubMed).

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