GFAP antibody (AA 256-357)
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- Target See all GFAP Antibodies
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Binding Specificity
- AA 256-357
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GFAP antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against GFAP. It has been selected for its ability to recognize GFAP in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Glial Fibrillary Acidic Protein (GFAP) corresdonding to Trp256~Leu357 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product GFAP Primary Antibody
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- Application Notes
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Western blotting: 0.5-3 μg/mL
Immunohistochemistry: 5-30 μg/mL
Immunocytochemistry: 5-30 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Alternative Name
- Glial Fibrillary Acidic Protein (GFAP Products)
- Synonyms
- GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
- Background
- Intermediate Filament Protein
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