Goat anti-Human IgG (F(ab')2 Region) Antibody (TRITC) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- F(ab')2 Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Flow Cytometry (FACS), Immunomicroscopy (IM)
- Characteristics
- Molar Ratio: 2.0 moles TRITC per mole of Rabbit IgG.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Human IgG F(ab')2 fragment.
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- Application Notes
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Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Comment
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Absorption / Emission: 550 nm / 570 nm
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide, 0.01 % Thimerosal as preservative.
- Preservative
- Sodium azide, Thimerosal (Merthiolate)
- Precaution of Use
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WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
This product also contains thimerosal which is hazardous. - Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Product is photosensitive and should be protected from light. - Storage
- 4 °C
- Storage Comment
- Store vial at 4 °C prior to restoration.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Human Immunoglobulin G
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