IL-10RA antibody

Catalog No. ABIN1177268

Product Details anti-IL-10RA Antibody

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of the activity elicited by recombinant mouse IL-10. Neutralization activity may be measured with a proliferation assay with 1x10^5 cells/mL D36 indicator cells using 6 ng/mL recombinant mouse IL-10 (Cat. No. 550070) costimulated with 50 pg/mL recombinant mouse IL-4 (Cat. No. 550067) (i.e preincubatiion of the antibody with the cells for 60 min)
50% Neutralization (ND50) at 0.1 - 1 ng/mL
>95% Neutralization at ≥ 1 - 15 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Purified recombinant ligand-binding domain of mIL-10R

Clone: 1B1-3a

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-10RA antibody (ABIN1177268)

OFarrell, Liu, Moore, Mui: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).

Target Details for IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: CD210 (IL10RA Products)

Synonyms: IL10R1 antibody, IL10RA antibody, CD210 antibody, CD210a antibody, CDW210A antibody, HIL-10R antibody, IL-10R1 antibody, IL10R antibody, AW553859 antibody, CDw210 antibody, CDw210a antibody, Il10r antibody, mIL-10R antibody, interleukin 10 receptor subunit alpha antibody, interleukin 10 receptor, alpha antibody, IL10RA antibody, Il10ra antibody

Background: The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R.
Synonyms: IL-10 Receptor

Pathways: Growth Factor Binding