RAD52 Homolog (S. Cerevisiae) (RAD52) antibody

Antigen: RAD52 Homolog (S. Cerevisiae) (RAD52)

Synonyms: Rad52yh, RAD52, MGC112400, zgc:112400, LOC100223085, MGC115453

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN117934

Additional Information

Alternative name: RAD52

UniProt: P43351

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52protein. AA Sequence: Human RAD52, 419 aa, predicted MW 46. 3 kDa: 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy

Cross-Reactivity: Human

Description: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52,a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate theDNA-DNA interaction necessary for the annealing of complementary DNA strands. It wasalso found to interact with DNA recombination protein RAD51, which suggested its role inRAD51 related DNA recombination and repair. Four alternatively spliced transcript variantsencoding different isoforms have been reported for this gene. The alpha variant encodesthe longest isoform that contains an identical N-terminus, but a distinct C-terminus, ascompared to other isoforms (beta, delta, and gamma).

Characteristics: Synonyms: DNA repair protein RAD52 homolog

Application Details

Application Notes: This affinity purified antibody has been tested for use in Western blotting (1: 500-1: 2,000)using HeLa nuclear extracts. The antibody is also functional by ELISA (1: 5,000-1: 20,000). Dilutions for Western blotting represent a starting point dilution and further optimizationmay be required. The antibody detects a band of approximately 63.0 kDa (predictedmolecular weight: 46.3 kDa). Specific band detection by Western blot is blocked bypeptide competition by pre-incubating the antibody with the immunizing peptide prior toreaction with the membrane. Reactivity in other immunoassays is unknown.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: Affinity Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis is shown using anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Publications

Muris, Bezzubova, Buerstedde et al.: "Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination." in: Mutation research, Vol. 315, Issue 3, pp. 295-305, 1994 (PubMed).

Park: "Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15467-70, 1995 (PubMed).

Zou, Yang, Chu et al.: "[Distribution of CSF1PO, TPOX and TH01 loci in Han Chinese]" in: Yi chuan xue bao = Acta genetica Sinica, Vol. 25, Issue 3, pp. 199-204, 1998 (PubMed).