RAD52 antibody

Catalog No. ABIN7216752

The Rabbit Polyclonal anti-RAD52 antibody (ABIN7216752) specifically detects RAD52 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for RAD52 antibody (ABIN7216752)

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD52 Antibody

Purpose: Rabbit Anti-Rad52 Polyclonal Antibody

Specificity: Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rad52 around the non-phosphorylation site of Y104

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: Rad52

Background: RAD52, DNA repair protein RAD52 homologRAD52 homolog, DNA repair protein encoded by RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. RAD52 product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of RAD52 is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of RAD52 have been described, but their full-length nature is not known.

Molecular Weight: 46kD

Gene ID: 5893

UniProt: P43351

Pathways: DNA Damage Repair