BMI1 antibody (AA 252-264)

Catalog No. ABIN118020

Product Details anti-BMI1 Antibody

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Binding Specificity: AA 252-264

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against human Bmi1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Orangutan, Canine, Chimpanzee, Froq, Bovine, Cat, Xenopus and Chicken.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 252-264 of Human Bmi1 protein.

Application Details

Application Notes: ELISA: 1/5,000-1/30,000Western blot: 1/500-1/3,000. Expect a band approx. 37 kDa in size corresponding to Bmi1 in theappropriate cell lysate or extract. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 % (w/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1 / RNF51 (BMI1 Products)

Synonyms: FLVI2/BMI1 antibody, PCGF4 antibody, RNF51 antibody, AW546694 antibody, Bmi-1 antibody, Pcgf4 antibody, bmi1 antibody, pcgf4 antibody, psc1 antibody, wu:fb17g03 antibody, wu:fd18f06 antibody, BMI-1 antibody, pcgf4b antibody, bmi-1 antibody, bmi1-a antibody, bmi1-b antibody, bmi1b antibody, rnf51 antibody, xbmi-1 antibody, BMI1 proto-oncogene, polycomb ring finger antibody, BMI1 polycomb ring finger oncogene antibody, Bmi1 polycomb ring finger oncogene antibody, bmi1 polycomb ring finger oncogene 1a antibody, bmi1 polycomb ring finger oncogene 1b antibody, BMI1 proto-oncogene, polycomb ring finger L homeolog antibody, BMI1 antibody, LOC100230513 antibody, Bmi1 antibody, bmi1a antibody, bmi1b antibody, bmi1.L antibody

Background: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gene ID: 100532731

NCBI Accession: NP_001190991

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy