Images for product: anti-alpha-2-HS-Glycoprotein (AHSG) antibody
Immunoblot of Fetuin..,.Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by immunoblot against fetuin in purified preparations and in human plasma..,.Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ul of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by.,.immunoblot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye™800 conjugated Donkey anti-Goat IgG for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similarresults.