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Details for Product No. ABIN118142

Integrin, alpha M (Complement Component 3 Receptor 3 Subunit) (ITGAM) antibody (FITC)

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Antigen
Reactivity
»Alternatives Human, Mouse (Murine), Rabbit
Host
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 7 references available
Catalog no. ABIN118142
Quantity 0.1 mg
Price
242.00 $   Plus shipping costs $45.00
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Immunogen T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Clone M1-70-15
Isotype IgG2b
Specificity This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
Purification Affinity chromatography on Protein A
Alternative Name CD11b
Background MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Alternate names: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
NCBI Accession 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: Neat - 1/10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Springer, Galfré, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Welt, Edelman, Simon et al.: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000 (PubMed).

Klingel, Schnorr, Sauter et al.: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Alternatives for antigen "Integrin, alpha M (Complement Component 3 Receptor 3 Subunit) (ITGAM)", type "Antibodies"
Hosts (123), (109), (27), (3), (2)
Reactivities (197), (136), (38), (18), (13), (13), (12), (7), (4), (3), (2), (1), (1)
Applications (196), (60), (44), (23), (21), (18), (15), (14), (13), (12), (10), (3), (3), (2), (1), (1)
Conjugates (49), (30), (25), (13), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (7), (4), (2), (1), (1), (1)
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