Integrin alpha M (ITGAM) antibody

Details for Product No. ABIN118146
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Antigen
Synonyms CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
Reactivity
Human, Mouse (Murine), Rabbit
(354), (306), (64), (21), (15), (13), (13), (6), (6), (3), (2), (1), (1)
Host
Rat
(282), (276), (55), (7), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(110), (78), (66), (29), (13), (8), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(515), (117), (112), (77), (63), (40), (37), (37), (31), (25), (10), (9), (7), (6), (3), (3), (2), (1), (1), (1)
Pubmed 18 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN118146
253.00 $
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Immunogen T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA Rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Clone M1-70-15
Isotype IgG2b
Purification Affinity Chromatography on Protein G
Alternative Name CD11b
Background MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: 1/50 - 1/200. Immunoprecipitation. Immunohistochemistry on frozen and paraffin sections: Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Integrin alpha M (ITGAM) antibody Staining of mouse peritoneal macrophages with rat anti mouse CD11b (ABIN118146).
Background publications Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Springer, Galfré, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Welt, Edelman, Simon et al.: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000 (PubMed).

Klingel, Schnorr, Sauter et al.: "beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis." in: The American journal of pathology, Vol. 162, Issue 5, pp. 1709-20, 2003 (PubMed).

Kapturczak, Wasserfall, Brusko et al.: "Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse." in: The American journal of pathology, Vol. 165, Issue 3, pp. 1045-53, 2004 (PubMed).

Hudcovic, Kozáková, Kolínská et al.: "Monocolonization with Bacteroides ovatus protects immunodeficient SCID mice from mortality in chronic intestinal inflammation caused by long-lasting dextran sodium sulfate treatment." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 1, pp. 101-10, 2009 (PubMed).

Huang, Hossain, Wu et al.: "Role of H2-calponin in regulating macrophage motility and phagocytosis." in: The Journal of biological chemistry, Vol. 283, Issue 38, pp. 25887-99, 2008 (PubMed).

Brochard, Combadière, Prigent et al.: "Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease." in: The Journal of clinical investigation, Vol. 119, Issue 1, pp. 182-92, 2009 (PubMed).

Samanta, Alden, Gobeske et al.: "Noggin protects against ischemic brain injury in rodents." in: Stroke; a journal of cerebral circulation, Vol. 41, Issue 2, pp. 357-62, 2010 (PubMed).

Ferger, Campanelli, Reimer et al.: "Effects of mitochondrial dysfunction on the immunological properties of microglia." in: Journal of neuroinflammation, Vol. 7, pp. 45, 2010 (PubMed).

Chinnery, Ruitenberg, McMenamin: "Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice." in: Journal of neuropathology and experimental neurology, Vol. 69, Issue 9, pp. 896-909, 2010 (PubMed).

Kanu, Penicud, Hristova et al.: "The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain." in: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38534-42, 2010 (PubMed).

Ghasemlou, Lopez-Vales, Lachance et al.: "Mitogen-activated protein kinase-activated protein kinase 2 (MK2) contributes to secondary damage after spinal cord injury." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 41, pp. 13750-9, 2010 (PubMed).

Redensek, Rathore, Berard et al.: "Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury." in: Glia, Vol. 59, Issue 4, pp. 603-14, 2011 (PubMed).

Geier, Celli: "Phagocytic receptors dictate phagosomal escape and intracellular proliferation of Francisella tularensis." in: Infection and immunity, Vol. 79, Issue 6, pp. 2204-14, 2011 (PubMed).

General Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

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