TNFRSF4 antibody

Catalog No. ABIN118274

Product Details anti-TNFRSF4 Antibody

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.

Purification: Affinity chromatography on Protein G

Immunogen: CHO cells transfected with murine OX40. Spleen cells from immunised AO rats were fused with cells of the mouse NS1 myeloma cell line.

Clone: OX-86

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TNFRSF4

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: CD134 / TNFRSF4 (TNFRSF4 Products)

Background: The OX40 receptor is also known as CD134, ACT35, and tumour necrosis factor receptor superfamily member 4. This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX40 receptor binds to the OX40 ligand to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, imply that these interactions are important in the pathogenesis of some autoimmune diseases.Synonyms: ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L, Tumor necrosis factor receptor superfamily member 4

Gene ID: 22163

NCBI Accession: NP_035789

UniProt: P47741

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints