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CD3 antibody (CD3) (FITC)

Details for Product anti-CD3 Antibody No. ABIN118478, Supplier: Log in to see
Antigen
  • CD3
Reactivity
Human, Primate
742
198
72
42
32
25
19
17
13
10
10
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
2
1
Host
Mouse
819
138
59
47
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3 antibody is conjugated to FITC
154
106
88
67
22
21
19
17
16
16
15
15
14
11
10
8
7
7
6
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
828
201
169
106
104
104
103
76
56
43
19
12
11
10
10
9
7
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
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Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Clone UCHT1
Isotype IgG1
Specificity This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19 kDa. As a pan T cell marker this antibody is present on more than 95 % of circulating human peripheral T cells.
Purification Affinity Chromatography on Protein G.
Alternative Name CD3 (CD3 Antibody Abstract)
Background CD3 is a surface protein of peripheral blood T cells of molecular weight 19 kD. The pan T cell marker is present on more than 95 % of circulating human peripheral T cells.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells or 100 μL of lysed whole peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This antibody is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-CD3 antibody (CD3)  (FITC) (ABIN118478) ABIN118478 staining of human peripheral blood lymphocytes.
Background publications Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).