CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (RPA-T4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (IHC)

Catalog no.: ABIN118486

Additional Information

Alternative name: CD4

UniProt: P01730

Immunogen: Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line

Cross-Reactivity: Human

Endotoxin Level: Low Endotoxin

Isotype: IgG1

Clone: RPA-T4

Description: CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.

Specificity: This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation.

Application Details

Application Notes: Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.4

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).