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CD4 Molecule (CD4) antibody

Details for Product No. ABIN118486
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Antigen
Synonyms cd4, L3T4, Ly-4, CD4mut, W3/25, p55
Reactivity
Human
(600), (451), (143), (43), (33), (25), (25), (24), (20), (20), (10), (6), (4), (3), (3), (3), (1)
Host
Mouse
(677), (388), (163), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(177), (129), (116), (69), (35), (17), (11), (10), (9), (9), (8), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Flow Cytometry (FACS)
(927), (209), (171), (163), (136), (125), (94), (55), (48), (44), (38), (12), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 12 references available
Quantity 0.5 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN118486
632.50 $
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Immunogen Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone RPA-T4
Isotype IgG1
Specificity This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD4
Background CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.
Alternate names: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607.1
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt, store undiluted (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Wright, Jones, Puklavec et al.: "The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans." in: Immunology, Vol. 102, Issue 2, pp. 173-9, 2001 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Voehringer, Koschella, Pircher: "Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1)." in: Blood, Vol. 100, Issue 10, pp. 3698-702, 2002 (PubMed).

Zhang, de Lara, Worth et al.: "Accelerated in vivo proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism." in: PLoS pathogens, Vol. 9, Issue 4, pp. e1003310, 2013 (PubMed).

General Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human \T\" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody."" in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

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