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CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN118486, Supplier: Log in to see
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Reactivity
Human
926
591
204
60
42
25
23
21
20
15
13
9
8
7
7
6
5
5
3
3
2
2
2
2
1
1
Host
Mouse
1099
549
184
4
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
253
215
150
111
38
36
24
24
21
20
19
16
15
15
14
13
10
8
8
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1376
350
310
247
202
166
158
80
73
59
33
28
27
18
14
11
10
9
8
6
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone RPA-T4
Isotype IgG1
Specificity This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt, store undiluted (in aliquots) at -20 °C.
Background publications Zhang, de Lara, Worth et al.: "Accelerated in vivo proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism." in: PLoS pathogens, Vol. 9, Issue 4, pp. e1003310, 2013 (PubMed).

Voehringer, Koschella, Pircher: "Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1)." in: Blood, Vol. 100, Issue 10, pp. 3698-702, 2002 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Wright, Jones, Puklavec et al.: "The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans." in: Immunology, Vol. 102, Issue 2, pp. 173-9, 2001 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).