CD11b antibody

Catalog No. ABIN118516

Product Details anti-CD11b Antibody

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody recognizes the CD11b cell surface glycoprotein. Clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.

No Cross-Reactivity: Cat (Feline)

Cross-Reactivity (Details): Species reactivity (tested):Human, Cynomolgus Monkey , Rhesus Monkey, Baboon.

Purification: Affinity Chromatography on Protein G

Immunogen: Rheumatoid synovial cells and Human monocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Clone: ICRF44

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (use 10 μL of 1:10 - 1:100 diluted antibody to label 10^6 cells or 100 μL whole blood). Immunoprecipitation Immunohistochemistry on frozen sections (1:100 - 1:1000) Recommended positive control tissue: Human tonsil. Not suitable for paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.

References for anti-CD11b antibody (ABIN118516)

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, (2006) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background: CD11b is a 165 kD molecule also know as the alpha-M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta-2 integrin and is found on monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. ICR44 significantly blocks homotypic PNM aggregation on response to FMLP (3,4).Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation