Integrin beta 2 antibody

Catalog No. ABIN118554

Product Details anti-Integrin beta 2 Antibody

Target: Integrin beta 2 (ITGB2)

Reactivity: Human, Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the human CD18 cell surface antigen, the integrin beta2 subunit. This antibody blocks CD18 mediated homotypic adhesion.

Purification: Purified

Immunogen: Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.

Clone: YFC118-3

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/1000-1/5000. Recommended positive control tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2 (ITGB2 Products)

Background: CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 3689

NCBI Accession: NP_000202

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation