Did you know that you can buy products from over 140 different suppliers from us?

Intercellular Adhesion Molecule 1 (ICAM1) antibody

Details for Product No. ABIN118732
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms BB2, CD54, P3.58, Icam-1, Ly-47, MALA-2, ICAM, ICAM-1, ICAM1
Reactivity
Dog (Canine), Human
(487), (130), (87), (41), (33), (17), (17), (1)
Host
Mouse
(291), (233), (54), (6), (5), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(56), (48), (32), (11), (10), (10), (9), (8), (8), (8), (8), (8), (8), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(309), (223), (123), (105), (101), (100), (95), (60), (41), (32), (32), (16), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
Quantity 0.2 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN118732
555.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen K562 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Clone 84H10
Isotype IgG1
Specificity This antibody recognises the D1 domain of ICAM-1. It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD. Clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.
Purification Affinity Chromatography on Protein A
Alternative Name CD54 / ICAM1
Background ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
Alternate names: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
NCBI Accession 9606
UniProt P05362
Application Notes Flow Cytometry: Use 10 ul of Neat-1/10 diluted antibody to label 10e6 cells in 100 ul. Immunohistochemistry on Frozen Sections (1/20-1/50). Immunoprecipitation: 5 ug/10e7 cells. Removal of sodium azide is recommended prior to use in functional assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Berendt, McDowall, Craig et al.: "The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site." in: Cell, Vol. 68, Issue 1, pp. 71-81, 1992 (PubMed).

Makgoba, Sanders, Ginther Luce et al.: "ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells." in: Nature, Vol. 331, Issue 6151, pp. 86-8, 1988 (PubMed).

Simmons, Makgoba, Seed: "ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM." in: Nature, Vol. 331, Issue 6157, pp. 624-7, 1988 (PubMed).

Rothlein, Dustin, Marlin et al.: "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 4, pp. 1270-4, 1986 (PubMed).

Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).

General Dustin, Rothlein, Bhan et al.: "Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 1, pp. 245-54, 1986 (PubMed).

Validation Images
back to top