Platelet-Endothelial Tetraspan Antigen 3 (CD151) antibody

Antigen: Platelet-Endothelial Tetraspan Antigen 3 (CD151)

Synonyms: GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24

Clonality: Monoclonal (11G5a)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN118883

Additional Information

Alternative name: CD151 / TSPAN24

Swiss-Prot: P48509

Isotype: IgG1

Clone: 11G5a

Description: CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. Alternate Names: TSPAN24, TSPAN-24, PETA-3, PETA3, GP27, Membrane Glycoprotein SFA-1, Tetraspanin-24, Tetraspanin 24, Platelet-Endothelial Tetraspan Antigen 3.

Specificity: This antibody recognises the CD151 cell surface antigen, also known as PETA-3.

Application Details

Application Notes: ELISA. Flow cytometry: 1/50 - 1/100, use 10 ul of the suggested working dilution to label 10e6 cells or 100 ul of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions (ref.1).

Concentration: 1.0 mg/ml

Buffer: This antibody is supplied as liquid, Protein G affinity purified immunoglobulin fraction in PBS buffer with 0.09% sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Staining of human peripheral blood platelets with Mouse anti Human CD151 (ABIN118882).

Publications

Publications: Yauch, Berditchevski, Harler et al.: "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." in: Molecular biology of the cell, Vol. 9, Issue 10, pp. 2751-65, 1998 (PubMed).