CD151 Molecule (Raph Blood Group) (CD151) antibody (PE)
| Antigen |
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| Synonyms |
CD151, GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24, MGC83668, MGC128855, gp27, mer2, raph, sfa1, peta-3, tspan24 |
| Clonality |
Monoclonal (11G5a) |
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Host
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Reactivity
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Conjugate
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FITC (7), PE (5), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RPE (1)
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Application
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Immunofluorescence (IF) (31), Western Blotting (WB) (26), ELISA (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN118884 |
| Quantity |
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| Price |
418.00 $ Plus shipping costs $45.00
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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CD151 / TSPAN24
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UniProt
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P48509
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Immunogen
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Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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11G5a
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Description
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CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. (2) It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions. (1) Alternate Names: TSPAN24, TSPAN-24, PETA-3, PETA3, GP27, Membrane Glycoprotein SFA-1, Tetraspanin-24, Tetraspanin 24, Platelet-Endothelial Tetraspan Antigen 3.
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Application Notes
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Flow Cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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contains 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
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Storage
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Store the antibody undiluted Prior to and following reconstitution at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf Life: one year from despatch. Reconstitution: Restore with 1 ml distilled water.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Yauch, Berditchevski, Harler et al.: "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." in: Molecular biology of the cell, Vol. 9, Issue 10, pp. 2751-65, 1998 (PubMed).
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Alternatives for antigen "CD151 Molecule (Raph Blood Group) (CD151)", type "Antibodies"
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Hosts
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Rabbit (50), Mouse (21)
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Reactivities
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Human (68), Mouse (Murine) (40), Rat (Rattus) (39), Chicken (19), Cow (Bovine) (19), Horse (Equine) (18), Yeast (Saccharomyces cerevisiae) (4), Cat (Feline) (1), Dog (Canine) (1), Primate (1)
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Applications
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Immunofluorescence (IF) (31), Western Blotting (WB) (26), ELISA (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (IHC) (1)
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Conjugates
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FITC (7), PE (5), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RPE (1)
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Epitopes
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C-Term (20), pSer30 (19), AA 26-35 (2), AA 26-35,pSer30 (1), AA 45-62 (1), Extracellular Domain (1), N-Term (1)
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