KRT14 antibody (C-Term) (FITC)

Catalog No. ABIN118964

The Mouse Monoclonal anti-KRT14 antibody has been validated for IF and FACS. It is suitable to detect KRT14 in samples from Human.

Quick Overview for KRT14 antibody (C-Term) (FITC) (ABIN118964)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is conjugated to FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: LL002

Product Details anti-KRT14 Antibody (FITC)

Binding Specificity: C-Term

Specificity: Recognizes Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14. Mouse anti Cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011).

Purification: Affinity Chromatography on Protein A

Immunogen: Last 15 C-terminal residues of Human cytokeratin 14 conjugated to thyroglobulin.

Isotype: IgG3

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL (Requires Cell Permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Gene ID: 3861

NCBI Accession: NP_000517

UniProt: P02533