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KRT14 antibody (C-Term) (FITC)

KRT14 Reactivity: Human IF, FACS Host: Mouse Monoclonal LL002 FITC

Catalog No. ABIN118964

Datasheet as PDF

Target See all KRT14 Antibodies

KRT14 (Keratin 14 (KRT14))
Binding Specificity
25

15

9

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
C-Term
Reactivity
101

62

47

7

7

3

2

2

1

1

1

1

1

1
Human
Host
67

37

2
Mouse
Compatible Secondaries
Clonality
57

48

1
Monoclonal
Conjugate
64

6

5

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This KRT14 antibody is conjugated to FITC
Application
44

34

29

26

25

17

13

13

13

12

10

4

2

2

1

1
Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity

Recognizes Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14. Mouse anti Cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011).

Purification

Affinity Chromatography on Protein A

Immunogen

Last 15 C-terminal residues of Human cytokeratin 14 conjugated to thyroglobulin.

Clone

LL002

Isotype

IgG3

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anti-Keratin 14 (KRT14) antibody
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anti-Keratin 14 (KRT14) (AA 1-484) antibody
KRT14 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Keratin 14 (KRT14) (C-Term) antibody
KRT14 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Keratin 14 (KRT14) (C-Term) antibody
KRT14 Reactivity: Human, Rat, Mouse IHC, IF, FACS, StM Host: Mouse Monoclonal LL002 unconjugated

anti-Keratin 14 (KRT14) (AA 351-472) antibody
KRT14 Reactivity: Human, Rat, Mouse, Pig IHC, FACS, StM Host: Mouse Monoclonal KRT14-2375 unconjugated

anti-Keratin 14 (KRT14) antibody
KRT14 Reactivity: Human, Rat, Pig, Dog WB, IHC, FACS, ICC, IHC (fro) Host: Mouse Monoclonal RCK107 unconjugated

anti-Keratin 14 (KRT14) (C-Term) antibody
KRT14 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Keratin 14 (KRT14) antibody
KRT14 Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated

anti-Keratin 14 (KRT14) (AA 276-303) antibody
KRT14 Reactivity: Human, Mouse WB, IHC, ELISA, FACS Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry: Use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL (Requires Cell Permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

KRT14 (Keratin 14 (KRT14))

Alternative Name

Cytokeratin 14 (KRT14 Products)

Background

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Gene ID

3861

NCBI Accession

NP_000517

UniProt

P02533