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Antibodies
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anti-Cytokeratin 18 (KRT18) antibody (Biotin)
Cytokeratin 18 (KRT18) antibody (Biotin)
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Clonality |
Monoclonal (DC-10) |
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Host
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Reactivity
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Human (123), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (9), Hamster (5), None (4), Sheep (Ovine) (3), Cat (Feline) (2), Bird (Avian) (1), Monkey (1)
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Conjugate
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FITC (13), Biotin (10), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (68), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (64), Flow Cytometry (FACS) (63), Immunofluorescence (IF) (57), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), ELISA (19), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (13), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN118967 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
290.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Cytokeratin 18
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Swiss-Prot
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P05783
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Immunogen
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Human breast carcinoma cell line PMC-42
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Isotype
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IgG1
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Clone
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DC-10
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Description
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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
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Specificity
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The antibody clone DC-10 reacts with Cytokeratin 18 (45 kDa).
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Application Notes
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ELISA. Western blotting. Immunohistochemistry (on Paraffin sections). Immunofluorecence. Suggested working dilution is 1:500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS containing 15 mM sodium azide, approx. pH 7.4
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Storage
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Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Signaling, Cytoskeleton, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Rabbit (75), Mouse (69), Guinea Pig (1)
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Reactivities
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Human (123), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (9), Hamster (5), None (4), Sheep (Ovine) (3), Cat (Feline) (2), Bird (Avian) (1), Monkey (1)
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Applications
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Western Blotting (WB) (68), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (64), Flow Cytometry (FACS) (63), Immunofluorescence (IF) (57), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (23), ELISA (19), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (13), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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FITC (13), Biotin (10), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitopes
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pSer33 (7), C-Term (5), Center (1), N-Term (1)
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