You are viewing an incomplete version of our website. Please click to reload the website as full version.

TLR4 antibody (Toll-Like Receptor 4) (FITC)

Details for Product anti-TLR4 Antibody No. ABIN119329, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Show less
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
(358), (116), (43), (20), (17), (17), (17), (12), (7), (1), (1)
(214), (187), (25), (8)
Clonality (Clone)
Monoclonal ()
This TLR4 antibody is conjugated to FITC
(38), (38), (38), (12), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS)
(244), (223), (98), (95), (70), (58), (46), (40), (24), (12), (12), (11), (8), (8), (6), (4), (3), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Specificity This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Purification Affinity Chromatography on Protein G.
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
Gene ID 7099, 9606
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Pathways TLR Signaling
Application Notes Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 l whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-TLR4 antibody (Toll-Like Receptor 4)  (FITC) (ABIN119329) Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC (ABIN1...
Background publications Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Catalog No. ABIN119329
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)