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Toll-Like Receptor 4 (TLR4) antibody (FITC)

Antigen

Toll-Like Receptor 4 (TLR4)
Synonyms TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8
Clonality Monoclonal (HTA125)
Host
Alternatives

Mouse
Reactivity
Alternatives

Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Conjugate
Application
Alternatives Flow Cytometry (FACS)
2 references available
Catalog no. ABIN119329
Quantity 0.1 mg  (0.1 mg/ml)  (Variants)
Price 429.00 $   Plus shipping costs $45.00
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anti-Toll-Like Receptor 4 (TLR4) antibody (FITC)

Additional Information
Application Details Publications Images Alternatives Variants back to top

Alternative name CD284 / TLR4
Gene ID 7099
UniProt O00206
Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0myeloma cell line.
Cross-Reactivity Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Isotype IgG2a
Clone HTA125
Description TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on humanmonocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence ofthe MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated followingstimulation with LPS.
Characteristics Synonyms: Toll-like receptor 4

Application Details
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Application Notes Flow Cytometry: Use 10 l of neat antibody to label 10e6 cells or 100 l whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Buffer PBS containing 0.09% Sodium Azide as preservative and 1% BSA asstabilizer.Label: Fluorescein Isothiocyanate Isomer 1 (FITC).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Signaling, Immunology, Inflammation
Restrictions For Research Use only

Publications
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Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts Mouse (91), Rabbit (88), Rat (10), Goat (4)
Reactivities Human (161), Mouse (Murine) (52), Rat (Rattus) (32), Pig (Porcine) (30), Dog (Canine) (27), Cow (Bovine) (24), Sheep (Ovine) (19), Monkey (12), Guinea Pig (8), Chimpanzee (3), Horse (Equine) (3), Macaque (2), Bison (Bison) (1)
Applications Flow Cytometry (FACS) (100), Western Blotting (WB) (87), ELISA (39), Immunoprecipitation (IP) (35), Immunofluorescence (IF) (24), Immunohistochemistry (IHC) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunocytochemistry (ICC) (16), Functional Studies (Func) (13), Neutralization (Neut) (7), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (2), Blocking Antibody (Inhibition) (1), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates FITC (19), Biotin (17), PE (13), Alexa Fluor (4), Alexa Fluor 647 (4), APC (2), Alexa Fluor 488 (2), RPE (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Cy7,PE (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (12), AA 100-200 (7), C-Term (5), AA 420-435 (3), Internal Region (3), AA 650-700 (2), AA 100-150 (1), AA 161-192 (1), AA 39-56 (1), AA 6-169 (1), Center (1), Extracellular Domain (1)