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Toll-Like Receptor 4 (TLR4) antibody

Antigen

Toll-Like Receptor 4 (TLR4)
Synonyms TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8
Clonality Monoclonal (HTA125)
Host
Alternatives

Mouse
Reactivity
Alternatives

Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
2 references available
Catalog no. ABIN119330
Quantity 0.5 mg  (1.0 mg/ml)  (Variants)
Price 605.00 $   Plus shipping costs $45.00
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anti-Toll-Like Receptor 4 (TLR4) antibody

Additional Information
Application Details Publications Images Alternatives Variants back to top

Alternative name CD284 / TLR4
UniProt O00206
Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Cross-Reactivity Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Endotoxin Level Low Endotoxin
Isotype IgG2a
Clone HTA125
Description TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Alternate Names: TLR4.

Application Details
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Application Notes Flow Cytometry: 1/10 - 1/50, Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Immunoprecipitation. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Concentration 1.0 mg/ml
Buffer PBS
Storage Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Signaling, Immunology, Inflammation
Restrictions For Research Use only

Publications
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Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts Rabbit (97), Mouse (91), Rat (10), Goat (4)
Reactivities Human (170), Mouse (Murine) (55), Rat (Rattus) (32), Pig (Porcine) (29), Dog (Canine) (26), Cow (Bovine) (24), Sheep (Ovine) (19), Monkey (12), Guinea Pig (8), Chimpanzee (3), Horse (Equine) (3), Macaque (2), Bison (Bison) (1)
Applications Flow Cytometry (FACS) (102), Western Blotting (WB) (95), ELISA (44), Immunoprecipitation (IP) (34), Immunohistochemistry (IHC) (25), Immunofluorescence (IF) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunocytochemistry (ICC) (19), Functional Studies (Func) (12), Neutralization (Neut) (7), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Blocking Antibody (Inhibition) (1), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates FITC (20), Biotin (17), PE (13), Alexa Fluor (4), Alexa Fluor 647 (4), APC (2), Alexa Fluor 488 (2), RPE (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Cy7,PE (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (16), AA 100-200 (11), C-Term (8), Internal Region (6), AA 420-435 (5), AA 650-700 (5), AA 39-56 (2), Center (2), AA 100-150 (1), AA 161-192 (1), AA 6-169 (1), AA 976-998 (1), Extracellular Domain (1), Internal Region,AA 676-706 (1), pTyr674 (1)