anti-TLR4 antibody (Toll-Like Receptor 4)

Details for Product anti-TLR4 Antibody No. ABIN119330
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Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Reactivity
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
(359), (122), (38), (21), (21), (21), (17), (13), (10), (1), (1)
Host
Mouse
(206), (204), (25), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
This TLR4 antibody is un-conjugated
(42), (41), (40), (14), (7), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(273), (219), (99), (92), (77), (64), (39), (36), (22), (12), (11), (10), (8), (7), (4), (4), (3), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.5 mg
Shipping to United States ( )
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Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Purification Affinity chromatography on Protein G
Endotoxin Level < 0.01 EU/ug
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
NCBI Accession NM_138554
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
anti-Toll-Like Receptor 4 (TLR4) antibody Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD284: LOW ENDOTOX...
Background publications Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

General Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Validation Images
Catalog No. ABIN119330
632.50 $
Plus shipping costs $45.00
Quantity
Price
0.5 mg
632.50 $

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