KIT antibody

Catalog No. ABIN119401

Product Details anti-KIT Antibody

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human, Monkey, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes the CD117 cell surface antigen. This antibody is Non-Neutralising.

Purification: Affinity Chromatography

Immunogen: MOLM-1 megakaryocyte cell line.

Clone: 104D2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/2 - 1/20, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 / C-Kit (KIT Products)

Background: C-kit, also known as stem cell factor receptor, steel factor receptor or CD117 is classified as a type III receptor tyrosine kinase (RTK) belonging to the platelet-derived growth factor receptor subfamily. Binding of stem cell factor (SCF), known as c-kit ligand to c-kit initiate autophosphorylation of the receptor, subsequently leading to promotes a signal transduction cascade through Ras-Raf-MAP kinase cascade, phosphatidylinositol-3-kinase, src family kinases, and STATs. The role of c-kit includes maturation of hematopoietic and primordial germ cells precursors and melanocytes during embryonic development. In acute myeloid leukemia (AML), c-kit has been proposed to play a functional role, and becomes target molecule for drug development.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR

Gene ID: 3815

NCBI Accession: NP_000213

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity