CD163 antibody (CD163 Molecule)

Details for Product anti-CD163 Antibody No. ABIN120083, Supplier: Log in to see
Antigen
  • DMBT1
  • CD163
  • CD163v2
  • CD163v3
  • M130
  • MM130
  • deleted in malignant brain tumors 1 protein-like
  • CD163 molecule
  • CD163 antigen
  • LOC426820
  • CD163
  • Cd163
Reactivity
Human, Monkey, Sheep (Ovine)
336
73
42
33
16
15
15
15
9
1
1
1
1
Host
Mouse
319
104
Clonality (Clone)
Monoclonal ()
Conjugate
This CD163 antibody is un-conjugated
37
36
34
13
11
11
9
7
7
7
7
5
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
250
222
143
107
99
89
69
56
55
13
13
8
5
4
4
3
3
2
Options
Supplier
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Immunogen Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.
Clone EDHu-1
Isotype IgG1
Specificity This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163.
Cross-Reactivity (Details) Species reactivity (tested):Human, Rhesus Monkey, Sheep.
Purification Affinity Chromatography on Protein A
Alternative Name CD163 (CD163 Antibody Abstract)
Background CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
Gene ID 9332
NCBI Accession NP_004235
UniProt Q86VB7
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Application Notes Flow Cytometry. Immunohistochemistry on frozen sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Vinet-Oliphant, Alvarez, Buza, Borda, Mohan, Aye, Tuluc, Douglas, Lackner: "Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS." in: The American journal of pathology, Vol. 177, Issue 3, pp. 1286-97, 2010 (PubMed).

Taus, Schneider, Oaks, Yan, Gailbreath, Knowles, Li: "Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo." in: Veterinary microbiology, Vol. 145, Issue 1-2, pp. 47-53, 2010 (PubMed).

Grund, Schittenhelm, Roser, Tatagiba, Mawrin, Kim, Bornemann: "The microglial/macrophagic response at the tumour-brain border of invasive meningiomas." in: Neuropathology and applied neurobiology, Vol. 35, Issue 1, pp. 82-8, 2009 (PubMed).

Moreno, Muñoz-García, Martín-Ventura, Madrigal-Matute, Orbe, Páramo, Ortega, Egido, Blanco-Colio: "The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis." in: Atherosclerosis, Vol. 207, Issue 1, pp. 103-10, 2009 (PubMed).

Jensen, Schmidt, Møller, Høyer, Maniecki, Sjoegren, Christensen, Steiniche: "Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 20, pp. 3330-7, 2009 (PubMed).

Fabriek, Polfliet, Vloet, van der Schors, Ligtenberg, Weaver, Geest, Matsuno, Moestrup, Dijkstra, van den Berg: "The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor." in: Blood, Vol. 109, Issue 12, pp. 5223-9, 2007 (PubMed).

Kim, Alvarez, Fisher, Bronfin, Westmoreland, McLaurin, Williams: "CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood." in: The American journal of pathology, Vol. 168, Issue 3, pp. 822-34, 2006 (PubMed).

Keh, Onyekwelu, McManus, McGuigan: "Corrosive injury to upper gastrointestinal tract: Still a major surgical dilemma." in: World journal of gastroenterology : WJG, Vol. 12, Issue 32, pp. 5223-8, 2006 (PubMed).

Montes de Oca, Torres, De Sanctis, Mata, Hernández, Tálamo: "Skeletal muscle inflammation and nitric oxide in patients with COPD." in: The European respiratory journal, Vol. 26, Issue 3, pp. 390-7, 2005 (PubMed).

Madsen, Møller, Nielsen, Jacobsen, Graversen, van den Berg, Moestrup: "Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 51561-7, 2004 (PubMed).

Kristiansen, Graversen, Jacobsen, Sonne, Hoffman, Law, Moestrup: "Identification of the haemoglobin scavenger receptor." in: Nature, Vol. 409, Issue 6817, pp. 198-201, 2001 (PubMed).