Integrin beta 2 antibody (Biotin)

Catalog No. ABIN120418

Product Details anti-Integrin beta 2 Antibody (Biotin)

Target: Integrin beta 2 (ITGB2)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This Antibody is specific for the β subunit of LFA-1. This antibody also recognizes MAC-1 and p150, 95 as well as LFA-1, since they all contain the same β subunit. It inhibits homeotypic aggregation of PHA blasts and blocks the binding of rat lymphocytes to purified rat ICAM-1

Clone: WT-3

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Flow cytometry. Immunohistochemistry on cryostat sections. Functional studies: in vivo/in vitro.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2, with 0.09 % NaN3 and 0.5 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2 (ITGB2 Products)

Background: LFA-1 (lymphocyte function associated molecule-1) is one of the leukocyte integrins. It is a heterodimer consisting of α and β subunits of 160-170 kDa and 95-100 kDa respectively. LFA-1 promotes non-antigen dependent adhesion of T-cells to a variety of lymphoid cells that bear its complementary receptor I-CAM-1 (1). It has a broad distribution and is found on most common lymphocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 25021

NCBI Accession: NP_036843

UniProt: Q9JI30

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation