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Integrin beta 2 antibody

ITGB2 Reactivity: Rat FACS, WB, IP, Func Host: Mouse Monoclonal WT-3 unconjugated

Catalog No. ABIN120420

Datasheet as PDF

Target See all Integrin beta 2 (ITGB2) Antibodies

Integrin beta 2 (ITGB2)
Reactivity
192

53

42

9

6

3

3

2

2

2

2

2

2

2

1

1
Rat
Host
99

87

35

2

1

1
Mouse
Compatible Secondaries
Clonality
131

89

2
Monoclonal
Conjugate
100

22

20

16

8

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This Integrin beta 2 antibody is un-conjugated
Application
129

81

51

44

37

32

28

26

26

24

22

12

8

3

3

3

2

2

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Specificity

This antibody reacts with the beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. Clone WT.3 has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1. SM264A is recommended for this purpose.

Purification

Affinity chromatography on Protein G

Immunogen

FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Clone

WT-3

Isotype

IgG1

Top Product

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anti-Integrin beta 2 (ITGB2) antibody
ITGB2 Reactivity: Human FACS, WB, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal MEM-48 unconjugated

anti-Integrin beta 2 (ITGB2) antibody
ITGB2 Reactivity: Human FACS, WB, IP, IHC (fro), Func, IHC (p) Host: Mouse Monoclonal MEM-48 unconjugated

anti-Integrin beta 2 (ITGB2) antibody (FITC)
ITGB2 Reactivity: Human FACS Host: Mouse Monoclonal MEM-48 FITC

anti-Integrin beta 2 (ITGB2) antibody (Biotin)
ITGB2 Reactivity: Human FACS, WB, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal MEM-48 Biotin

anti-Integrin beta 2 (ITGB2) antibody (PE)
ITGB2 Reactivity: Human FACS Host: Mouse Monoclonal MEM-48 PE

anti-Integrin beta 2 (ITGB2) (AA 70-170) antibody
ITGB2 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Integrin beta 2 (ITGB2) antibody
ITGB2 Reactivity: Mouse FACS, WB, IP, IHC (fro), Func, IHC (p) Host: Rat Monoclonal M18-2 unconjugated

anti-Integrin beta 2 (ITGB2) (AA 559-700) antibody
ITGB2 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 3C11G5 unconjugated

anti-Integrin beta 2 (ITGB2) (AA 17-382) antibody
ITGB2 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Integrin beta 2 (ITGB2) antibody
ITGB2 Reactivity: Mouse FACS, WB, IP, IHC (fro), IHC (p) Host: Rat Monoclonal M18-2 unconjugated

Application Notes

Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.2 containing 0.09 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Integrin beta 2 (ITGB2)

Alternative Name

CD18 / ITGB2 (ITGB2 Products)

Background

CD18 is expressed by all leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID

25021

NCBI Accession

NP_036843

UniProt

Q9JI30

Pathways

NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation