CD43 antibody

Catalog No. ABIN120446

The Mouse Monoclonal anti-CD43 antibody is suitable to detect CD43 in samples from Rat. It has been validated for FACS, IHC (p) and IHC (fro).

Quick Overview for CD43 antibody (ABIN120446)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: W3-13

Product Details anti-CD43 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Protein G Chromatography

Immunogen: Rat thymocyte membrane glycoproteins

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL, this product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections and paraffin sections (requires antigen retrieval using heat treatment prior to staining).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.2 with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 / Leukosialin

Background: CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gene ID: 10116

UniProt: P13838

Pathways: Regulation of Leukocyte Mediated Immunity