CD45 antibody (PE)

Catalog No. ABIN120466

This Mouse Monoclonal antibody specifically detects CD45 in FACS. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for CD45 antibody (PE) (ABIN120466)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: OX-1

Product Details anti-CD45 Antibody (PE)

Specificity: This antibody recognises CD45. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.

Immunogen: Rat thymocyte membrane glycoproteins.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-CD45 antibody (PE) (ABIN120466)

Eipel, Bordel, Nickels, Menger, Vollmar: "Impact of leukocytes and platelets in mediating hepatocyte apoptosis in a rat model of systemic endotoxemia." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 286, Issue 5, pp. G769-76, (2004) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 / LCA

Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gene ID: 24699

NCBI Accession: NP_001103357

UniProt: P04157

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling