CD45 antibody (FITC)

Catalog No. ABIN120484

This Mouse Monoclonal antibody specifically detects CD45 in FACS. It exhibits reactivity toward Rat.

Quick Overview for CD45 antibody (FITC) (ABIN120484)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: OX-22

Product Details anti-CD45 Antibody (FITC)

Specificity: This antibody recognises CD45RC, the high molecular weight form of the leucocyte common antigen.

Purification: Affinity chromatography on Protein G

Immunogen: PHA stimulated rat lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: NEAT - 1/10, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 / LCA

Background: The antigen is found on B cells, approximately 50 % of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gene ID: 24699

NCBI Accession: NP_001103357

UniProt: P04157

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling