This antibody recognizes the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend SM286LE for this use.
Cross-Reactivity (Details)
Species reactivity (tested):Rat.
Purification
Affinity Chromatography on Protein G
Immunogen
Rat Ax cells (a rat HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
Flow cytometry (Use 10 μL neat antibody to label 10^6 cells in 100 μL). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (see Ref.3). Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend the use of ABIN182014 for this purpose. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL IgG
Buffer
PBS, pH 7.2, containing 1 % BSA as stabiliser and 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD54 cell surface antigen, also known as the intercellular adhesion molecule-1 (ICAM-1), is a 90 kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor