FYN antibody (AA 7-176)

Catalog No. ABIN120706

This Mouse Monoclonal antibody specifically detects FYN in WB, IF, IP and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for FYN antibody (AA 7-176) (ABIN120706)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FYN-01

Product Details anti-FYN Antibody

Binding Specificity: AA 7-176

Specificity: The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.

Purification: Protein A Affinity Chromatography

Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176)

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation (Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting (2 mg/mL, Positive control: JURKAT human T cell leukemia cell line). Immunocytochemistry. Immunohistochemistry (paraffin sections): 5 m g/mL. Positive tissue: brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Expiry Date: 12 months

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN

Background: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

Gene ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling