FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 7-176)

Details for Product anti-FYN Antibody No. ABIN94311
Antigen
Epitope
AA 7-176
30
29
11
10
10
10
10
10
8
7
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
218
133
79
7
5
5
4
4
4
4
4
2
2
1
Host
Mouse
175
57
8
Clonality (Clone)
Monoclonal ()
Conjugate
This FYN antibody is un-conjugated
13
9
8
7
7
7
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
209
129
63
33
24
19
17
10
5
4
3
2
2
2
Options
Immunogen Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
Clone FYN-01
Isotype IgG2b
Specificity The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.
Cross-Reactivity (Details) Mouse, Human, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Fyn (FYN Antibody Abstract)
Background Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats...
Image no. 2 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (; gre...
Image no. 3 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn
Image no. 4 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (...
Product cited in: Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).