LAT2 antibody (AA 91-244)

Catalog No. ABIN120727

This Mouse Monoclonal antibody specifically detects LAT2 in WB, IP, FACS and IF. It exhibits reactivity toward Human and Mouse. It has been mentioned in 3+ publications

Quick Overview for LAT2 antibody (AA 91-244) (ABIN120727)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: NAP-07

Product Details anti-LAT2 Antibody

Binding Specificity: AA 91-244

Specificity: The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant cytoplasmic domain (aa 91-244) of human NTAL

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-LAT2 antibody (ABIN120727)

Nørsett, Laegreid, Langaas, Wörlund, Fossmark, Waldum, Sandvik: "Molecular characterization of rat gastric mucosal response to potent acid inhibition." in: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, (2007) (PubMed).

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: LAT2 / WBSCR15

Background: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein

Gene ID: 7462

UniProt: Q9GZY6

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling