Linker For Activation of T Cells Family, Member 2 (LAT2) antibody

Antigen: Linker For Activation of T Cells Family, Member 2 (LAT2)

Synonyms: LAB, NTAL, WSCR5, WBSCR5, HSPC046, WBSCR15, LAT2, MGC139435

Clonality: Monoclonal (NAP-07)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN120727

Additional Information

Alternative name: LAT2 / WBSCR15

UniProt: Q9GZY6

Immunogen: Recombinant cytoplasmic domain (aa 91-244) of human NTAL

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: NAP-07

Description: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Specificity: The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Application Details

Application Notes: Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/ml, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Publications

Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).