Crystallin, alpha A (CRYAA) antibody

Antigen: Crystallin, alpha A (CRYAA)

Synonyms: CRYA1, HSPB4, Crya1, lop18, Acry-1, Crya-1, DAcry-1, AI323437, cryaa-A, CRYAA, fc06h04, MGC92036, zgc:92036, wu:fc06h04, alpha-a-crystallin

Clonality: Monoclonal (C9F2)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN121144

Additional Information

Alternative name: Alpha-crystallin A chain

Swiss-Prot: P02489

Immunogen: Recombinant human a -Crystallin A protein

Isotype: IgG1  (Matching secondary antibodies)

Clone: C9F2

Description: A -crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products a -A and a -B, for acidic and basic, respectively. a -crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of a -crystallins, and the a -A and a -B subunits have a 3:1 ratio, respectively. Two additional functions of a -crystallins are an autokinase activity and the participation in the intracellular architecture. Alternate Names: Alpha-crystallin A chain, Heat shock protein beta-4, HSPB4, CRYA1, Heat shock protein beta 4, Alpha-A crystallin, Zonular Central Nuclear Cataract, Crystallin Alpha 1.

Specificity: The antibody recognizes human and rat a -Crystallin A. Other species not tested.

Application Details

Application Notes: ELISA. Western blot (1:500 - 1:2,000), recommended starting dilution 1:1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 0.09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Neurology, Enzymes

Restrictions: For Research Use only

Images

Immunoblot analysis: Rat eye extracts were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-crystallin alphaalpha(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates crystalline alphaalpha(~20 kDa).

Publications

Publications: Laksanalamai, Robb: "Small heat shock proteins from extremophiles: a review." in: Extremophiles : life under extreme conditions, Vol. 8, Issue 1, pp. 1-11, 2004 (PubMed).