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CRYAA antibody (Biotin)

CRYAA Reactivity: Human WB, ELISA, IF, ICC Host: Mouse Monoclonal 1H3-B8 Biotin
Catalog No. ABIN2481844
  • Target See all CRYAA Antibodies
    CRYAA (Crystallin, alpha A (CRYAA))
    Reactivity
    • 45
    • 17
    • 16
    • 3
    • 1
    Human
    Host
    • 28
    • 17
    • 1
    Mouse
    Clonality
    • 28
    • 18
    Monoclonal
    Conjugate
    • 21
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CRYAA antibody is conjugated to Biotin
    Application
    • 34
    • 26
    • 16
    • 10
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~20 kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Native Alpha Crystallin
    Clone
    1H3-B8
    Isotype
    IgG1
    Top Product
    Discover our top product CRYAA Primary Antibody
  • Application Notes
    • WB (1:2000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.5 μg/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    CRYAA (Crystallin, alpha A (CRYAA))
    Alternative Name
    Alpha A Crystallin (CRYAA Products)
    Synonyms
    CRYA1 antibody, CTRCT9 antibody, HSPB4 antibody, AI323437 antibody, Acry-1 antibody, Crya-1 antibody, Crya1 antibody, DAcry-1 antibody, lop18 antibody, alpha-a-crystallin antibody, cryaa-A antibody, CRYAA antibody, wu:fc06h04 antibody, zgc:92036 antibody, HspB4 antibody, crystallin alpha A antibody, crystallin, alpha A antibody, alphaA-crystallin antibody, alpha-crystallin A chain-like antibody, crystallin alpha A S homeolog antibody, alpha-crystallin A chain antibody, CRYAA antibody, Cryaa antibody, CpipJ_CPIJ005646 antibody, cryaa antibody, LOC100384978 antibody, cryaa.S antibody, LOC458589 antibody, LOC102724652 antibody
    Background
    The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).
    Gene ID
    1409
    NCBI Accession
    NP_000385
    UniProt
    P02489
    Pathways
    M Phase
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