IRF5 antibody (AA 176-240)

Catalog No. ABIN121152

Cited in 1 publication. The Mouse Monoclonal anti-IRF5 antibody (Clone 10T1) (ABIN121152) specifically detects IRF5 in WB, IP, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for IRF5 antibody (AA 176-240) (ABIN121152)

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 10T1

Product Details anti-IRF5 Antibody

Binding Specificity: AA 176-240

Specificity: The antibody recognizes Human and Mouse Interferon Regulatory Factor 5 (IRF-5). Other species not tested.

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human IRF-5 (176-240 aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot (1/500 - 1/1,000). Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-IRF5 antibody (ABIN121152)

Idborg, Zandian, Ossipova, Wigren, Preger, Mobarrez, Checa, Sohrabian, Pucholt, Sandling, Fernandes-Cerqueira, Rönnelid, Oke, Grosso, Kvarnström, Larsson, Wheelock, Syvänen, Rönnblom, Kultima et al.: "Circulating Levels of Interferon Regulatory Factor-5 Associates With Subgroups of Systemic Lupus Erythematosus Patients. ..." in: Frontiers in immunology, Vol. 10, pp. 1029, (2019) (PubMed).

Target Details for IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Alternative Name: IRF5

Background: IRF-5 is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs(IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.Synonyms: IRF-5, Interferon regulatory factor 5

Gene ID: 3663

UniProt: Q13568

Pathways: TLR Signaling, Autophagy