Cyclin A antibody

Catalog No. ABIN121516

This Rabbit Polyclonal antibody specifically detects Cyclin A in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin A antibody (ABIN121516)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Cyclin A Antibody

Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 60 kDa Cyclin A protein from Human, Rat and Mouse tissue. No reaction was observed against other related Cyclins. Cross reactivity with Cyclin A from other species may also occur.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat and Mouse.

Purification: Delipidation and defibrination.

Immunogen: Full length GST fusion protein corresponding to the human cyclin A gene

Application Details

Application Notes: Immunoprecipitation. ELISA: 1/500-1/2000. Western blot: 1/250-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 80 mg/mL (by Refractometry)

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A2

Background: Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA