Western Blot: 0.2 μg/mL - 1 μg/mL, this antibody detects a band of approximately 130 kDa in HeLa, Jurkat and NSO (mouse) cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
TRIS buffered saline pH 7.3 containing 0.02 % Sodium Azide and 0.5 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date
12 months
Target
DDB1
(Damage Specific DNA Binding Protein 1 (DDB1))
Alternative Name
DDB1 / XAP1
Background
DDB1 is a 130kD protein that forms the large subunit of DNA damage binding protein (DDB). DDB1 is involved in the nucleotide-excision repair of DNA following damage caused by ultraviolet radiation.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein