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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (C-Term) antibody
|Synonyms||MCH3, CMH-1, ICE-LAP3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, mch3, cmh-1, ice-lap3, CASP7, LOC100101591|
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (1.0 mg/ml) (Variants)|
|Price||418.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Immunogen||A 16 amino acid peptide from human Caspase-7 carboxy-terminus|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||ICE-LAP3 is a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADPribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways. Alternate Names: CASP7, Mch-3, ICE-LAP3, CMH-1, MCH3, Caspase 7, CASP 7, ICE-like apoptotic protease 3, ICE- LAP3, Apoptotic protease Mch-3, apoptosis-related cysteine protease, ICELAP3, CMH1.|
|Specificity||This antibody recognises an epitope within the C-terminal region (CT) of Caspase-7, a 35kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) Caspase-7.|
|Application Notes||Western Blot: 1.0ug/ml - 2.0ug/ml. Immunohistochemistry on paraffin sections: 2.0ug/ml, this product does not require protein digestion pre-treatment of paraffin sections eg. Trypsin or pronase prior to staing, this product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Skeletal muscle tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 0.02% Sodium Azide|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.|
|Restrictions||For Research Use only|
Duan, Chinnaiyan, Hudson et al.: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).
Juan, McNiece, Argento et al.: "Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3." in: Genomics, Vol. 40, Issue 1, pp. 86-93, 1997 (PubMed).
Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).