UBE2N antibody (C-Term)

Catalog No. ABIN121903

This Rabbit Polyclonal antibody specifically detects UBE2N in WB and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for UBE2N antibody (C-Term) (ABIN121903)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBE2N Antibody

Binding Specificity: C-Term

Purification: Ion exchange chromatography

Immunogen: A 15 amino acid peptide located near the human UBC13 carboxy-terminus

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 μg/mL - 1 μg/mL, this antibody detects a band of approximately 16 kDa in human small intestine cell lystates. Immunohistochemistry on frozen sections: 2 μg/mL, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections, sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Mouse thymus tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Background: UBC13 is a 16 kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation.Synonyms: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway