Chemokine (C-C Motif) Receptor 7 (CCR7) (C-Term) antibody

Antigen: Chemokine (C-C Motif) Receptor 7 (CCR7)

Synonyms: BLR2, EBI1, CD197, CDw197, CMKBR7, Ebi1h, Cdw197, Cmkbr7, MGC108519, CCR7, MGC165629, zgc:165629

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN122618

Additional Information

Alternative name: CD197 / CCR7 (C-term)

Swiss-Prot: P32248

Immunogen: Synthetic peptide - KLH conjugated

Description: CCR7, a Chemokine Receptor, is involved in lymphocyte recirculation through secondary lymphoid organs and moving lymphocytes and dendritic cells into distinct tissue compartments. CCR7 is called the Epstein-Barr-induced receptor because it mediates Epstein- Barr viral effects on B lymphocytes. CCR7 expression is up-regulated in mature dendritic cells, B cells infected with EBV, and in CD4+ cells infected with human herpes virus 6 and 7. Immature dendritic cells have been shown to express chemokine receptor 5 (CCR5) and migrate in response to macrophage inflammatory protein-1alpha (MIP-1alpha), whereas mature dendritic cells switch to CCR7 expression and respond exclusively to MIP-3beta and 6Ckine. Maturing dendritic cells upregulate CCR7, which drives their migration to the T-cell areas of the draining lymph nodes where antigen is presented to naive T cells. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. This receptor is particularly expressed in lymphoid tissues and in activated B and T lymphocytes. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries Alternate Names: CDW197, CMKBR7, EBI1, EVI1, BLR2, CC-CKR-7, blr2, c-c chemokine receptor 7, cc chemokine receptor 7, chemokine c-c motif receptor 7, cmkbr7, ebi1, epstein-barr virus induced g-protein coupled receptor, lymphocyte-specific g protein-coupled peptide receptor.

Specificity: Reacts with CC Chemokine Receptor 7 (CCR7) (family: GPCR, subfamily: Chemokine).The immunizing peptide shows 63 % identity (89 % similarity) with mouse and 68 % identity (94 % similarity) with rat

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 6-8 ug/ml Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Immunohistochemical staining of Lymph Node using anti- CCR7 antibody ABIN122618