CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (MEM-115)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN125679

Additional Information

Alternative name: CD4

Immunogen: Human thymocytes and T lymphocytes.

Format: Azide Free

Isotype: IgG2a  (Matching secondary antibodies)

Clone: MEM-115

Description: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains.Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity: The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDatransmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) andalso on monocytes, tissue macrophages and granulocytes. It is negative in Western blottingeven with non-reduced samples of cell lysates.HLDA V, WS Code T T-CD04.09

Application Details

Application Notes: Immunoprecipitation: excellent. Functional Application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also stronglyinhibits CD4-MHC Class II interactions. Flow CytometryApplication notes: Although it has not been tested rigorously, following data suggest that the antibodyMEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature,monovalent Fab fragments essentially do not bind to T cells.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Singer, Scott, Kawka et al.: "CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells." in: Journal of virology, Vol. 75, Issue 8, pp. 3779-90, 2001 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Bosze, Caccamo, Majer et al.: "In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis." in: Biopolymers, Vol. 76, Issue 6, pp. 467-76, 2004 (PubMed).