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Antibodies
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anti-Integrin alpha M (ITGAM) antibody
Integrin alpha M (ITGAM) antibody
| Antigen |
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| Synonyms |
CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam |
| Clonality |
Monoclonal (MEM-174) |
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Host
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Reactivity
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Human (108), Mouse (Murine) (90), Rat (Rattus) (20), Rabbit (18), Monkey (10), Cow (Bovine) (8), Dog (Canine) (6), Goat (4), Primates (3), Sheep (Ovine) (3), Cat (Feline) (1), Horse (Equine) (1), Pig (Porcine) (1)
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Flow Cytometry (FACS) (164), Immunoprecipitation (IP) (47), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Functional Studies (Func) (15), Immunohistochemistry (IHC) (14), Blocking Antibody (Inhibition) (13), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (3), ELISA (2), Immunofluorescence (IF) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN125683 |
| Quantity |
0.1 mg (1 mg/ml) (Variants) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD11b
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Immunogen
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Human granulocytes
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Format
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Azide Free
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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MEM-174
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Description
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CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein thatnon-covalently associates with integrin beta2 subunit (CD18), expression of the CD11bchain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin(Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less onmacrophages. CD11b/CD18 integrin is implicated in various adhesive interactions ofmonocytes, macrophages and granulocytes, facilitating their diapedesis, as well as itmediates the uptake of complement coated particles, serving as a receptor for the iC3bfragment of the third complement component.
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Specificity
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The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. TheCD11b mediates neutrophil and monocyte interactions with stimulated endothelium.HLDA VI, WS Code BP 310HLDA VI, WS Code M 18
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Application Notes
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Flow Cytometry - Positive control: Peripheral Blood Lymphocytes. Immunoprecipitation - Positive control: GranulocytesCD11b/CD18 transfectants. Application note: Immunoprecipitation with the antibody MEM-174 was successfulyperformed using immuno-affinity sorbents (minicolumns). The final immunosorbent shouldcontain 1-5 mg of antibody/1 ml of sorbent gel (e. g. agarose beads) - It is recommended to stimulate positive control material by LPS 1-2 days before to achievebetter expression of target antigen).
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by protein A-affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 ยต m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Publications
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Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).
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Alternatives for antigen "Integrin alpha M (ITGAM)", type "Antibodies"
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Hosts
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Mouse (103), Rat (84), Goat (6), Rabbit (6)
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Reactivities
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Human (108), Mouse (Murine) (90), Rat (Rattus) (20), Rabbit (18), Monkey (10), Cow (Bovine) (8), Dog (Canine) (6), Goat (4), Primates (3), Sheep (Ovine) (3), Cat (Feline) (1), Horse (Equine) (1), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (164), Immunoprecipitation (IP) (47), Immunohistochemistry (Frozen Sections) (IHC (fro)) (46), Functional Studies (Func) (15), Immunohistochemistry (IHC) (14), Blocking Antibody (Inhibition) (13), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (5), Immunocytochemistry (ICC) (3), ELISA (2), Immunofluorescence (IF) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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FITC (37), PE (23), Biotin (22), APC (7), RPE (4), SPRD (4), APC,Cy7 (3), Cy5 (2), PE,Cy7 (2), Alexa Fluor 647 (1), Alexa Fluor 700 (1), PE,Cy5 (1), PE,DY647 (1), PE-Dyomics 647 (1), RPE,Cy5 (1)
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