CD29 antibody (APC)

Antigen: CD29

Clonality: Monoclonal (MEM-101A)

Host: Mouse

Reactivity: Human, Pig (Porcine), Dog (Canine)

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN125692

Additional Information

Immunogen: Raji Burkitt's lymphoma cell line

Isotype: IgG1

Clone: MEM-101A

Description: CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties ofbeta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, lamininand other extracellular matrix (ECM) components. This interaction hinders cell death,whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of mostbeta1 integrins correlates with acquiring multidrug resistance of tumour cells duringselection in presence of antitumour drug. In platelets, translocation of intracellular pool ofbeta1 integrins to the plasma membrane following thrombin stimulation. These integrins arealso up-regulated in leukocytes during emigration and extravascular migration and appear tobe critically involved in regulating the immune cell trafficking from blood to tissue, as well asin regulating tissue damage and disease symptoms related to inflammatory bowel disease.Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhancecytokine secretion of human airway smooth muscle in response to IL-1beta.

Specificity: The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chaintype I glycoprotein expressed as a heterodimer (non-covalently associated with the integrinalpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic andnon-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cellsand mast cells).HLDA VI, WS Code AS A048 This antibody does not react with: Mouse .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with cross-linked Allophyc

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A). Lane 1: lysate of human PBL. Lane 2: lysate of canine PBL. Lane 3: lysate of porcine PBL

Publications

Publications: Kim, Cho: "Regulatory role of ginsenoside Rp1, a novel ginsenoside derivative, on CD29-mediated cell adhesion." in: Planta medica, Vol. 75, Issue 4, pp. 316-20, 2009 (PubMed).

Handke, Oelschlegel, Franke et al.: "Hantaan virus triggers TLR3-dependent innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2849-58, 2009 (PubMed).

Lomakina, Knauf, Schultz et al.: "Activation of human neutrophil Mac-1 by anion substitution." in: Blood cells, molecules & diseases, Vol. 42, Issue 3, pp. 177-84, 2009 (PubMed).

Soler, Chapman, Yang et al.: "The binding specificity and selective antagonism of vedolizumab, an anti-alpha4beta7 integrin therapeutic antibody in development for inflammatory bowel diseases." in: The Journal of pharmacology and experimental therapeutics, Vol. 330, Issue 3, pp. 864-75, 2009 (PubMed).

Wagner, Bork, Horn et al.: "Aging and replicative senescence have related effects on human stem and progenitor cells." in: PLoS ONE, Vol. 4, Issue 6, pp. e5846, 2009 (PubMed).

Byeon, Choi, Hong et al.: "Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses." in: Archives of pharmacal research, Vol. 32, Issue 6, pp. 813-22, 2009 (PubMed).

Lee, Lee, Cho: "Cell-permeable ceramides act as novel regulators of U937 cell-cell adhesion mediated by CD29, CD98, and CD147." in: Immunobiology, 2009 (PubMed).